FastQCFastQC Report
Tue 27 Oct 2020
ddSeq5_S4_L001-004_R2_rev.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameddSeq5_S4_L001-004_R2_rev.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172518245
Sequences flagged as poor quality0
Sequence length75
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32568251.8878148221366384No Hit
GATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA2095860.12148628105972212Illumina RNA PCR Primer (100% over 30bp)
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAG1754220.10168315820741163Illumina RNA PCR Primer (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)